Electrostatic interactions drive the self - assembly and the transcription activity of the 1 Pseudomonas phage φ 6 procapsid
نویسندگان
چکیده
Electrostatic interactions drive the self-assembly and the transcription activity of the 1 Pseudomonas phage φ6 procapsid 2 3 Xiaoyu Sun, Dennis H. Bamford, Minna M. Poranen 4 5 Department of Biosciences and Institute of Biotechnology, University of Helsinki, Viikki Biocenter, 6 Helsinki, Finland 7 8 Running title: Electrostatic interactions drive phage φ6 assembly 9 10 Abstract word count: 71 11 Text word count: 1345 12 13 #Address correspondence to Minna M. Poranen, [email protected] 14 JVI Accepts, published online ahead of print on 9 April 2014 J. Virol. doi:10.1128/JVI.00467-14 Copyright © 2014, American Society for Microbiology. All Rights Reserved.
منابع مشابه
Electrostatic interactions drive the self-assembly and the transcription activity of the Pseudomonas phage ϕ6 procapsid.
Assembly of an empty procapsid is a crucial step in the formation of many complex viruses. Here, we used the self-assembly system of the double-stranded RNA bacteriophage ϕ6 to study the role of electrostatic interactions in a scaffolding-independent procapsid assembly pathway. We demonstrate that ϕ6 procapsid assembly is sensitive to salt at both the nucleation and postnucleation steps. Furthe...
متن کاملRescue of maturation off-pathway products in the assembly of Pseudomonas phage φ 6.
Many complex viruses use an assembly pathway in which their genome is packaged into an empty procapsid which subsequently matures into its final expanded form. We utilized Pseudomonas phage 6, a well-established virus assembly model, to probe the plasticity of the procapsid maturation pathway. The 6 packaging nucleoside triphosphatase (NTPase), which powers sequential translocation of the three...
متن کاملElectrostatic interactions govern both nucleation and elongation during phage P22 procapsid assembly.
Icosahedral capsid assembly is an example of a reaction controlled solely by the interactions of the proteins involved. Bacteriophage P22 procapsids can be assembled in vitro by mixing coat and scaffolding proteins in a nucleation-limited reaction, where scaffolding protein directs the proper assembly of coat protein. Here, we investigated the effect of the buffer composition on the interaction...
متن کاملElectrostatic interactions drive scaffolding/coat protein binding and procapsid maturation in bacteriophage P22.
The first step in assembly of the bacteriophage P22 is the formation of a T=7 icosahedral "procapsid," the major components of which are the coat protein and an inner core composed of the scaffolding protein. Although not present in the mature virion, the scaffolding protein is required for procapsid assembly. Eleven amino-acid residues at the extreme carboxyl terminus of the scaffolding protei...
متن کاملThe effect of N- or C-terminal alterations of the connector of bacteriophage phi29 DNA packaging motor on procapsid assembly, pRNA binding, and DNA packaging.
Double-stranded DNA viruses package their genomes into procapsids via an ATP-driven nanomotor. This ingenious motor configuration has inspired the development of biomimetics in nanotechnology. Bacteriophage varphi29 DNA-packaging motor has been a popular tool in nanomedicine. To provide information for further motor modification, conjugation, labeling, and manufacturing, the connector protein g...
متن کامل